ABSTRACT
In researcher’s previous study it has been found that the 80% ethanolic extract of Daucus carota canopy L was the most active extract for antibacterial activity and its phytochemical screening led to the isolation and identification of 12 natural flavone compounds including the new C-glycoside; 6,8-Di-C-α-L-rhamnoside diosmetin besides 11 known compounds. The primary objective of the present work is to investigate the 80% EtOH extract of Daucus carota canopy L for its antioxidant, analgesic and cytotoxic activities and the new compound for its cytotoxic activity. The ethanolic extract (300 µL, 1 mg/ml) gave the highest effective antioxidant activity with inhibition % 82.09 ± 0.15 using radical scavenging method which is more than the standard BHA (68.2) and less than the standard TBHQ (98.2). The cytotoxic activity of ethanolic extract against MCF7 gave IC50 19.4 µg/ml, and the new compounds had IC50 is 8.05 µg/ml compare to Cisplatin which its IC50 is 5.48 µg/ml. Its anti-nociceptive properties show that the ethanolic extract (200 µL, 1 mg/ml) significantly (p<0.05) decreased the number of writhes in acetic acid writhing test and increased the response time in hot plate method when comparing with the control group.